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CABRI:DSMZ_MUTZ |
Accession_number |
ACC 413
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Cell_line_name |
639-V
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Brief_description |
confirmed as human with IEF of AST, MDH established from the transitional cell
carcinoma (small, grade III) removed from the left ureter of a 69-year-old white man in
1973
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Description |
human ureter transitional cell carcinoma established from the transitional cell
carcinoma (small, grade III) removed from the left ureter of a 69-year-old white man in
1973 confirmed as human with IEF of AST, MDH
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Depositor |
Dr. W. A. Schulz, Urologische Klinik, Duesseldorf, Germany
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Morphology |
epithelial-like cells growing adherently in monolayers
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Culture_conditions |
90% Dulbecco's MEM + 10% FBS split confluent culture 1:5 to 1:10 every 3-4 days
using trypsin/EDTA; seed out at ca. 0.5 x 106 cells/25 cm 2 at 37C with 5-10%
CO2 cell harvest of ca. 6 x 106 cells/25 cm 2
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Viruses |
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -,
SMRV -
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Properties |
cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +,
desmin -, endothel -, EpCAM -, GFAP -, neurofilament -, vimentin +
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Release_conditions |
-
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Hazard |
-
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Storage |
frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 106 cells/ampoule
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Doubling_time |
ca. 30 hours
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Mycoplasma |
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
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Fingerprint |
multiplex PCR of minisatellite markers revealed a unique DNA profile
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Cytogenetics |
human hyperdiploid karyotype with 24% polyploidy - 55(50-59)<2n>XX, +1, +3, +5,
+6, +8, +11, +12, +14, -15, +20, -21, add(1)(p?21), i(5p), del(6)(q13-14), i(7p),
add(8)(p2?), add(11)(p11), del(12)(q21q24), add(18)(q23) - additional copies of 6q-,
i(7p), 8p+, der(18) markers present in sidelines together with del(7)(p13-14)
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Bibliographic_reference |
Elliott et al., Cancer Res, 36: 35 (1976) [PMID: 944089
] |
Elliott et al., Cancer Res, 37: 35 (1977) [PMID: 66978]
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